Fig. 4: DE-MSC-mediated modulation of inflammatory responses in cell culture and mouse model.
From: Definitive Endodermal Cells Supply an in vitro Source of Mesenchymal Stem/Stromal Cells
a mRNA levels of proinflammatory cytokines (IL-6 and IL-8) and anti-inflammatory cytokines (IDO1 and PD-L1) were analyzed by RT-qPCR assay in DE-MSCs (FBS), DE-MSCs (CHIR), DE-MSCs (CHIR/SB), and UC-MSCs that were exposed to IFN-γ (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. b Effect of DE-MSCs (FBS) and UC-MSCs on anti-inflammatory cytokine TGF-β level in spleens of colitis mice, showing the anti-inflammatory effect of DE-MSCs (FBS) (n = 3). **P < 0.01. c Average changes in body weight of each group from day 0 to day 14 (3–5 mice in each group). The unit of body weight was the gram. d Images of colons dissected from mice in healthy mice, DSS + PBS, DSS + PBS + DE-MSCs (FBS), DSS + PBS + DE-MSCs (CHIR), DSS + PBS + DE-MSCs (CHIR/SB), and DSS + PBS + UC-MSCs groups on day 14 (n = 5). e The length of colons from multiple experiments described in (c) were measured and plotted (n = 5). **p < 0.01, ***p < 0.001. f Hematoxylin and eosin (H&E) staining of distal colon sections from healthy mice, DSS + PBS, DSS + PBS + DE-MSCs (FBS), DSS + PBS + DE-MSCs (CHIR), DSS + PBS + DE-MSCs (CHIR/SB) groups, scale bar = 100 μm.
