Fig. 3: Effects of CSS on population-level transcriptome expression of BA-NAc neurons. | Communications Biology

Fig. 3: Effects of CSS on population-level transcriptome expression of BA-NAc neurons.

From: Stress deficits in reward behaviour are associated with and replicated by dysregulated amygdala-nucleus accumbens pathway function in mice

Fig. 3

a Experimental design. Handling + BW: daily handling and measurement of body weight; S NAc: stereotactic surgery; CTB + Recovery: CTB transport and recovery from surgery; CSS/CON: CSS protocol or control handling; BP: brains were PBS perfused; LCM: collection of BA-NAc CTB+-labelled tissue using laser capture microdissection; RNA-seq: RNA sequencing and differential gene expression analysis. b Schematic showing bilateral injection site of retrograde CTB Alexa Fluor-555 in NAc. c Coronal image from mouse brain atlas62 at bregma level −1.8 mm with BA highlighted. d Representative coronal image (×5) from brain of a CSS mouse at bregma −1.8 mm before (left) and after (right) collection of CTB-555+ tissue samples using LCM. e Representative coronal image (×40) from a CSS mouse BA at bregma −1.8 mm: white circles indicate areas of CTB-555+ tissue demarcated for LCM. Scale bar = 200 µm. f Expression levels (transcript per million) of cell type-specific marker genes: Snap25 neuron, Slc17a7 glutamate neuron, Gad1 GABA neuron, Aqp4, astrocyte, Opalin myelinating oligodendrocyte, Pdgfra oligodendrocyte progenitor cell, Ctss microglia. g Volcano plot for differential gene expression in CSS compared with CON mice: significantly up-regulated genes are shown in red and significantly down-regulated genes in blue. Image b was created with BioRender.com. Image c was used with permission of Elsevier, from The Mouse Brain Atlas, G. Paxinos & K.B.J. Franklin, 2nd edition, 2001; permission conveyed through Copyright Clearance Center, Inc.

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