Fig. 5: Oxidized mtDNA binds to the pyrin domain of NLRP3.
From: Differential Binding of NLRP3 to non-oxidized and Ox-mtDNA mediates NLRP3 Inflammasome Activation
a EMSA for ox-DNA 20 bp. Anti-biotin DNA shift or anti-NLRP3 for FCAS mutant, WT, or DNA alone. b EMSA for ox-DNA 90 bp. Anti-biotin DNA shift or anti-NLRP3 for FCAS mutant, WT, or DNA alone.
