Fig. 6: NLRP3-DNA complex band intensity lost with anti-pyrin is recovered using a NACHT-targeting antibody.
From: Differential Binding of NLRP3 to non-oxidized and Ox-mtDNA mediates NLRP3 Inflammasome Activation
An EMSA was done using 90-bp biotinylated ox-mtDNA and four different NLRP3 constructs, L266F, NLRP3(1–93), NLRP3(1–134), and NLRP3(94–1034). a The same membrane is probed with an antibody targeting the biotinylated ox-DNA (left), an antibody targeting the pyrin domain (middle), and an antibody targeting the NACHT domain (right). b Graphs of the intensity of the bands from each respective membrane probe. In the NACHT-targeting graph, bound and free band intensities are relatively the same for the L266F and NLRP3(94–1034) constructs. In the pyrin-targeting graph, there is a roughly 80% decrease in the band intensity comparing the free L266F to the DNA-bound L266F. c Visual key for constructs used in this experiment.
