Fig. 5: Differential expression of Notch pathway components in TMA constructed from HNSCC patient tumors.
From: Identification of acquired Notch3 dependency in metastatic Head and Neck Cancer
4 μM sections of the TMA were stained with antibodies against the components of the Notch pathway previously identified as differentially expressed by metastatic HNSCC lines. a Distributions of fractions of positive cells for Jag1, Jag2, Hes1 and Hey1 across the TMA cores. Y-axis on the left axis indicates Density values, related to the density curve (the density curve is showing the smoothed distribution of the points along the x-axis). Y-axis on the right indicates frequency values related to the histogram. Thresholds between the subpopulations obtained by using EM algorithm (see “Methods”) are indicated with vertical black lines; the exact values of the thresholds are indicated near the corresponding lines. b Pearson’s Chi-squared tests were performed to examine the association between the pTstages (early and late) and the level of protein expression. Plots for Jagged1, Hes1 are reporting the p values for the general comparison (2 stages vs. 3 categories), the plot for Jagged2 reports the corrected p value for the specific comparison (intermediate/high vs. 2 stages) and the plot for Hey1 reports the p value for the comparison of high/low vs. 2 stages. c Proportion of cores that belong to high/low populations of Hey1 is calculated in each population of Jag2. The proportion of high Hey1 cores is higher in high Jag2 populations.
