Fig. 8: Inhibition of Notch signaling improves survival and inhibits growth of lymph node metastasis in an orthotopic mouse model of HNSCC.
From: Identification of acquired Notch3 dependency in metastatic Head and Neck Cancer
a UT-SCC-74A and UT-SCC-74B cells transduced with luciferase and with the inducible shRNA for Notch3 were injected submucosally into the tongues of the mice. After surgical removal of tongue tumors, images of lymph node metastasis of control (a) and doxycycline treated (b) mice were taken using the Xenogen IVIS-200 instrument. Numbers on top of the images represent number of days after the surgery. c The survival analysis of control and doxycycline treated mice are shown. Either poor health condition of the animal or luciferase signal of 1 × 109 at the site of metastasis was defined as an end point. Mantel-Cox test identified significant differences between the groups with improved survival of treated mice (p value 0.0087).
