Table. 1 List of resources used for data generation.
From: Identification of acquired Notch3 dependency in metastatic Head and Neck Cancer
Reagent or resource | Source | Identifier |
|---|---|---|
Antibodies | ||
Jag1 | Abcam | Cat# ab7771 RRID: AB_2280547 |
Jag2 | Abcam | Cat# ab109627 RRID: AB_10860796 |
Actin | Abcam | Cat# ab8227 RRID: AB_2305186 |
Hey1 | Novus | Cat# NBP2-47436 |
Hes1 | Novus | Cat# NBP2-67642 |
HRP anti-mouse | GE Healthcare | Cat# NA931, RRID: AB_772210 |
HRP anti-rabbit | GE Healthcare | Cat# GENA934, RRID: AB_2722659 |
Chemicals, peptides, and recombinant proteins | ||
Human Jagged1 (Ser32-Ser1046) | R&D systems | Accession # P78504 |
Human Jagged2 (Met27-Leu1082) | R&D systems | Accession #AAB61285 |
Deposited data | ||
Raw and analyzed data | This paper | GEO: GSE117753 |
Experimental Models: Cell Lines | ||
UT-SCC-54A | Gift of Dr. Grenman | RRID: CVCL_7863 |
UT-SCC-54C | Gift of Dr. Grenman | NA |
UT-SCC-60A | Gift of Dr. Grenman | RRID: CVCL_A089 |
UT-SCC-60B | Gift of Dr. Grenman | RRID: CVCL_A090 |
UT-SCC-74A | Gift of Dr. Grenman | NA |
UT-SCC-74B | Gift of Dr. Grenman | NA |
Experimental models: organisms/strains | ||
NSG mice | In house colony | IMSR Cat# NM-NSG-001, RRID: IMSR_NM-NSG-001 |
Sequence based elements | ||
See Supplementary data 10 for list of oligos | ||
Notch1 smart siRNA pool | Dharmacon | M-007771-02-0005 |
Notch3 smart siRNA pool | Dharmacon | M-011093-01-0005 |
Jag1 smart siRNA pool | Dharmacon | M-011060-02-0005 |
Jag2 smart siRNA pool | Dharmacon | M-017187-00-0005 |
EG5 smart siRNA pool | Dharmacon | M-003317-01-0005 |
Recombinant DNA | ||
TRIPZ Notch3 | Dharmacon | RHS4740-EG4854 |
pLenti CMV Puro Luc w168 | Addgene #17477 | |
pX458 | Addgene #48138 | |
H2B-GFP | Addgene #11680 | |
H2B-RFP | Addgene #26001 | |
Software and Algorithms | ||
siMEM | ||
muTect tool | ||
Oncotator | ||
MutSigCV tool | ||
