Fig. 1: Schematic overview of the RT-IVT method for IVT-mRNA quantification.
From: RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production
mRNA with the target site for the BFQ probes is transcribed from a DNA template. BFQ probes (BFQa and BFQb) carrying either a donor fluorophore or quencher fluorophore are present in the IVT mixture and hybridize to the mRNA. This brings the donor and quencher fluorophores into close proximity so that FRET can occur. The FRET signal allows quantification of mRNA concentration in real-time. Figure was created with Biorender (www.bioender.com).
