Fig. 3: Single-cell biophysical fractometry for drug response analysis of H2170 cells.
From: Morphological profiling by high-throughput single-cell biophysical fractometry
a Experimental workflow. b Randomly selected phase gradient images (∇ϕx, top), QPI (bottom) of H2170 cells treated with DTX, control and GCB, respectively. Scalar bar is 5 μm. c Statistical distributions of FD. d Statistical distributions of FD MSE2. e A fractal phenotypic profile of three different drug treatment (feature values are normalized based on the z-score). Each row represents a fractal feature, and each column represents a single cell. The scale bar stands for 1000 cells, which were randomly subsampled from each group. f ROC curves of the linear regression classifiers differing DTX/GCB-treated cells from control group, respectively, which were constructed from 17-dimension Fourier features. AUROC values are labeled in the legend. g 3D UMAP visualization of the ALS-related phenotypes extracted from single-cell images of drug-treated H2170 cells. Dispersion among different drug-treated clusters is observable.
