Fig. 5: Analysis of the single-cell phenotypic correlation among the fractal, ALS, and spatial features.
From: Morphological profiling by high-throughput single-cell biophysical fractometry
a A Circular plot summarizing the mean heatmap and the correlations among all features in cell-cycle progression. The feature type is labeled by the outmost colored ring (bulk–orange, global–blue, local–green, fractal–purple, and ALS–pink), and the full labels can be referred to Supplementary Fig. S6. The mean feature values of in different cell cycle phases are color-coded in the three ring-shaped heatmap. In the inner circle, all the feature pair with an absolute value of Spearman correlation coefficient over 0.6 are linked together by gray lines, while the Fourier-morphology connections are colored with orange specifically. Thickness of the lines is also encoded by the absolute value of correlation coefficient. b Spearman correlation heatmap between Fourier-domain and spatial-domain features. Fourier features are ranked by AUROC test. Spatial-domain features are ranked by the average of correlation coefficient with all Fourier features. The feature labels are colored according to the feature type specified in a. c Correlation coefficient bar chart of FD ranked by absolute value. Only 30 features with the largest coefficient magnitude with FD are listed.
