Fig. 5: In vivo biodistribution of HEK293 and Huh7 nPMVs and native EVs, DOPC:PS, and DOPC liposomes in the zebrafish larvae (ZFL). | Communications Biology

Fig. 5: In vivo biodistribution of HEK293 and Huh7 nPMVs and native EVs, DOPC:PS, and DOPC liposomes in the zebrafish larvae (ZFL).

From: High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications

Fig. 5: In vivo biodistribution of HEK293 and Huh7 nPMVs and native EVs, DOPC:PS, and DOPC liposomes in the zebrafish larvae (ZFL).

a Schematic representation of the experimental procedure: DiI labeled NPs were injected into the duct of Cuvier of two transgenic ZFL fish lines (kdrl:EGFP and mpeg1:Gal4:UAS:Kaede) 48 h post fertilization. 4 and 24 h post injection (hpi), tissue distribution in the tail region (red rectangle) was visualized by CLSM. b Light panel: Tissue distribution of DiI labeled NPs (red signal) in Tg(kdrl:EGFP) ZFL (green signal: vasculature). Scale bar: 100 µm. Insert: untreated ZFL. Right panel: 3D rendered orthogonal view of the left panel. Green signal: kdrl:EGFP. Red signal: DiI NPs. Scale bar: 100 µm. c Circulation factor (CF) is defined as the ratio between circulating (faint red) and bound (saturated red) NPs within the vasculature (green) of Tg(kdrl:EGFP) ZFL. Extravasation factor (EF) is the ratio between NP signal (red) inside and outside of the Tg(kdrl:EGFP) ZFL vasculature58. d Comparison of CF (left) and EF (right) between HEK293 and Huh7 nPMVs and native EVs, DOPC:PS, and DOPC liposomes 4 and 24 hpi in Tg(kdrl:EGFP) ZFL. Box plot: line: median, square: mean, box: lower and upper quartile, whisker: 1.5 interquartile range, filled square: outlier. n = 6 for HEK293 and Huh7 nPMVs and native EVs, n = 3 for DOPC:PS and DOPC liposomes. Levels of significance: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.

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