Fig. 3: The overview of the selected metabolic pathways in gunSOS1 compared with sigRep.

The map was constructed based on the measured metabolites presented in Supplementary Figures 5a, 6a, and 7. Metabolites increased in gunSOS1 compared to sigRep: fumaric acid (fumarate), oxoglutaric acid (2-OG), myo-inositol, ADP-glucose (ADPGlc), galactose 1-phosphate (Gal1P), fructose 1-phosphate (Fru1P), alpha-D-glucose 1,6-bisphosphate (Glc1, 6BP), glycerol 3-phosphate (Gly3P), phosphoenolpyruvic acid (PEP), and L-malic acid (malate). Metabolites decreased in gunSOS1 compared to sigRep: cis-aconitic acid (aconitate), mannose 6-phosphate (Man6P), glucose 6-phosphate (Glc6P), D-fructose (fructose), fructose 6-phosphate (Fru6P), glucose 1-phosphate (Glc1P), fructose 1,6-bisphosphate (Fru1, 6BP), and sorbitol. Metabolites that did not show significant difference in gunSOS1 relative to sigRep: D-glucose (glucose), D-maltose (maltose), maltotriose, citric acid (citrate), isocitric acid (isocitrate), pyruvic acid (pyruvate), succinic acid (succinate), and 3-phosphoglyceric acid (3PGA). The colour-coding is shown in the legend. Measurements were performed in biological triplicates (n = 3); significant differences were calculated using two-tailed Student’s t-test. The maximal significant differences from any time point in light (Supplementary Figures 5a and 6a) are indicated by asterisks, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.