Fig. 5: The qRT-PCR analysis of gene expression in gunSOS1 compared to sigRep. | Communications Biology

Fig. 5: The qRT-PCR analysis of gene expression in gunSOS1 compared to sigRep.

From: Singlet oxygen-induced signalling depends on the metabolic status of the Chlamydomonas reinhardtii cell

Fig. 5

a Expression of genes encoding protein components previously associated with the chloroplast retrograde signalling involving 1O2, PSBP215, MBS17, and SAK116. b Transcript levels of the selected genes showing attenuated expression in gunSOS1 compared to sigRep in the context of the SAK1-transcriptome16, genes description in the text. Experiments were performed in biological replications (n = 3); results are presented as a fold change (2-ΔΔCt, normalised to the mean of Ctexp-Ctref of WT); the error bars represent calculated ±SD. Significant differences were calculated using one-way ANOVA, pair-wise comparison with the Tukey’s post-hoc test (non-significant not shown), *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

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