Fig. 1: Previous knowledge on OGDHc and a morphological study of C. thermophilum, the model organism that is used to expand it.
From: Structural analysis of an endogenous 4-megadalton succinyl-CoA-generating metabolon
A Schematic representation of previous knowledge on OGDHc overall architecture. A cubic core comprised of 24 E2o proteins, with extended flexible linkers ending in the ordered LD domain, and E1o and E3 dimers on the periphery. B Complete reaction scheme of OGDHc. C Fluorescent light microscopy of isolated C. thermophilum filaments, with the cell membrane in red color, stained with FM464. D Fluorescent light microscopy of a single C. thermophilum filament, with the cell membrane in red color, stained with FM464. E Fluorescent light microscopy of a single C. thermophilum filament, with the highly abundant mitochondrial content visible in green color, stained with MiOr. F Transmission electron microscopy image of cryo-fixated ultra-thin sections of C. thermophilum filament. With “M” the mitochondria are annotated in the image. G Zoom-in into the mitochondria ultrastructures visible in F. The ribbon-like christae of the C. thermophilum mitochondria are visible (annotated as “RLC”).
