Fig. 3: The Cryo-EM structure of the OGDHc E2o 24-mer core.
From: Structural analysis of an endogenous 4-megadalton succinyl-CoA-generating metabolon
A The 3.35 Å (0.143 FSC) cryo-EM map of the C. thermophilum OGDHc E2o core. Scale bar: 5 nm. B The reconstructed model of the C. thermophilum E2o core, fitted in the cryo-EM map. Beneath, the inter- and intra-trimeric interfaces can be observed in isolation. C The CoA binding region of the E2o, with the annotated amino-acid sidechains that participate in its coordination. Additionally, resolved density in the pocket may correspond to a bound CoA. D Resolved density in the periphery of the E2o vertex trimer, possibly belonging to a bound E2o lipoyl domain.
