Fig. 1: SCT production and quality control. | Communications Biology

Fig. 1: SCT production and quality control.

From: Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis

Fig. 1

a Workflow of SCT production. Reduced SDS-PAGE gel showing expressed SCT protein (~50 kDa); SN supernatant, P His-Tag purified SCT. b Cell viability and transfection efficiency of SCT plasmid library. c SCT yield from each peptide element of the SCT plasmid library, with or without IRES-GFP reporter. Error bars represent standard deviation, n = 3 independent measurements. d Reduced SDS-PAGE gel of A*02:01 and A*24:02 SCTs pre- and post-PNGase treatment. NXT glycosylation motifs of peptides are underlined.

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