Fig. 4: Rescue of cell functions in the presence of idelalisib.
From: Functional impact and molecular binding modes of drugs that target the PI3K isoform p110δ
Ramos (a–c) or BaF3 (d) cells were retrovirally transduced and selected to stably express wild type or mutant p110δ. a Ramos cells expressing p110δ or p110δ-I777M were used to determine the anti-IgM induced secretion of the cytokines CCL3, CCL4, and TNFα in the presence of PI3Ki. Cytokine levels in culture supernatants were determined by ELISA. For each cytokine, three independent assays were performed. b, c The chemotaxis of isogenic BaF3 cells was examined in transwell migration assays. The numbers of migrated BaF3 cells expressing p110δ variants were compared in the absence and presence of the chemo-attractant CXCL12, as indicated by empty and filled symbols (b). Percentages of cells that migrated to CXCL12 in the absence or presence of 1 µM idelalisib were calculated from parallel experiments with passage-matched samples (c). Means and single values of four biological replicates are shown. P values were determined by two-sided unpaired T-tests.
