Fig. 4: Effects of BA.1 variant mutations on S1/S2 cleavage. | Communications Biology

Fig. 4: Effects of BA.1 variant mutations on S1/S2 cleavage.

From: SARS-CoV-2 omicron variants harbor spike protein mutations responsible for their attenuated fusogenic phenotype

Fig. 4: Effects of BA.1 variant mutations on S1/S2 cleavage.The alternative text for this image may have been generated using AI.

Huh7.5-A2T2 cells were transfected with SARS-CoV-2 variants S expressing vectors (a) or BA.1-associated mutants S expressing vectors (b). 24 h post-transfection, cells were collected with RIPA Lysis and Extraction Buffer and Protease Inhibitor Cocktail. The lysed samples were processed further to measure the S/S2 level with automated Western blot system (Simple Western™ Automated Western Blot System). Then, ImageJ was used to quantify the relative signal intensity. The β-actin-normalized relative signal intensities were plotted separately as S (Full), S2, and S2/S (Full) ratio. The data are representative of three independent experiments.

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