Fig. 2: Srr1 and Skb1 cause isochromosome formation but not chromosomal truncation.
From: Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere
a Depicted are the non-repetitive (cnt3) and repetitive sequences (the innermost imr3, dg, dh, and the outermost irc3) in cen3. The ura4+ marker gene is placed at 10 kb from cen3. Loss of Rad51 increases isochromosomes and chromosomal truncations. b Chromosomal DNAs prepared from the parental (P) and independent GCR clones of rad51∆, srr1∆ rad51∆, and skb1∆ rad51∆ strains (TNF5411, 5904, and 5788) were separated by PFGE. Sizes of lambda (λ) DNA ladders are indicated on the left of the panels. Sample numbers of isochromosomes and truncations are shown in blue and magenta, respectively. c Rates of isochromosome formation and chromosomal truncation in wild-type (TNF5369), rad51∆, srr1∆ rad51∆, skb1∆ rad51∆, and skb1-F319Y rad51∆ (TNF8391) strains. Rates relative to the rad51∆ strain are indicated on the top of the bars. The two-tailed Fischer’s exact test between the rad51∆ and other mutant strains. **p < 0.01; ****p < 0.0001. Numerical data underlying c are provided in Table B in Supplementary Data 1. Uncropped gel images are shown in Supplementary Fig. 5.
