Fig. 4: Srr1 plays a role in the Rad52-independent GCR pathway.
From: Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere
a GCR rates of wild-type, srr1∆, rad52-R45K, srr1∆ rad52-R45K, rad51∆, srr1-W157R rad51∆, rad52-R45K rad51∆, srr1-W157R rad52-R45K rad51∆, pcn1-K107R rad51∆, and srr1-W157R pcn1-K107R rad51∆ strains (TNF5369, 5774, 6599, 8281, 5411, 8344, 7122, 8663, 6761, and 8601). The two-tailed Mann-Whitney test. b Tetrad analysis of srr1∆ and rad52∆. srr1::kanR and rad52::hygR haploids (TNF5943 and 7988) were crossed, and the resulting tetrads were dissected on YE plates under a microscope. Images of three sets of three-spore viable tetrads in which the srr1::kanR rad52::hygR progenies did not form colonies are shown. c Depletion of Rad52 by the AID system impairs the growth of srr1∆ cells. rad52-AID, srr1∆ rad52-AID, OsTIRF74A, srr1∆ OsTIRF74A, rad52-AID OsTIRF74A, and srr1∆ rad52-AID OsTIRF74A (TNF8614, 8621, 8616, 8623, 8617, and 8627) were spotted on YE plates supplemented with 200 nM 5’a-IAA which induces Rad52 depletion. d Rpa2-mCherry foci (arrowhead) were observed by fluorescence microscopy in wild-type cells (TNF5492). Fluorescence and DIC images are overlayed. DIC, differential interference contrast. A bar shown below the image indicates 10 µm. The bar graph shows percentages of nuclei containing at least one Rpa2-mCherry focus in wild-type and srr1∆ (TNF8803) strains. The bars represent the mean of three independent experiments. The two-tailed student’s t-test. e Rad52-GFP foci (arrowhead) were observed in wild-type cells (TNF4442). The bar graph shows percentages of nuclei containing at least one Rad52-GFP focus in wild-type and srr1∆ (TNF6130) strains. Numerical data underlying a are provided in Table A, and those underlying d and e are in Table F in Supplementary Data 1.
