Fig. 5: A role of the SRR1-like domain in GCRs and DNA damage repair.
From: Fission yeast Srr1 and Skb1 promote isochromosome formation at the centromere
a Positions of the fission yeast srr1-D111A,P112A, -H148A, and -W157R mutation sites are indicated by blue circles. Similar and identical residues among the different species are highlighted in pale and dark gray, respectively. b A ribbon model of the Srr1 structure predicted by AlphaFold methods. Positions of the mutation sites are indicated. c A surface model of the Srr1 structure. Positively and negatively charged residues are shown in blue and red, respectively. d GCR rates of wild-type, rad51∆, srr1∆ rad51∆, srr1-W157R rad51∆, srr1-D111A,P112A rad51∆, and srr1-H148A rad51∆ (TNF5369, 5411, 5904, 8344, 8686, and 8387). The two-tailed Mann-Whitney test. Numerical data are provided in Table A in Supplementary Data 1. e Mutating the conserved residues in the SRR1-like domain increases the sensitivity to MMS, HU, and CPT. Wild-type, srr1∆, srr1-W157R, srr1-H148A, and srr1-D111A,P112A (TNF3885, 5847, 8280, 8275, and 8274) cells were spotted onto YE3S supplemented with the indicated concentrations of MMS, HU, or CPT.
