Fig. 1: miRNA/mRNA sequencing and analysis workflow. | Communications Biology

Fig. 1: miRNA/mRNA sequencing and analysis workflow.

From: Mouse microglia express unique miRNA-mRNA networks to facilitate age-specific functions in the developing central nervous system

Fig. 1: miRNA/mRNA sequencing and analysis workflow.The alternative text for this image may have been generated using AI.

Whole brains of three groups of C57Bl/6 mice (n = 12) were processed and CD45+ve microglia were isolated via immunopanning. Microglia and CD45−ve bulk cell populations were sequenced for miRNA expression and analysed for differential expression using the edgeR/limma-voom pipeline. In addition, mRNA expression was sequenced in microglial populations and normalised read counts were correlated with miRNA data to construct miRNA–mRNA coregulatory networks.

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