Fig. 2: Co-transport of Ca2+ and inorganic phosphate ion (Pi) by YfkE.
From: Ca2+ efflux facilitated by co-transport of inorganic phosphate anion in the H+/Ca2+ antiporter YfkE
a Time course of Pi transport of YfkE measured using inside-out vesicles (+/− 0.5 mM Ca2+). 5 mM 32Pi substrate was added to the reaction. Empty vesicles were used as control. b Coomassie-stained SDS-PAGE image of purified YfkE protein. c Assessment of the orientation of YfkE in proteoliposomes treated with thrombin. Immunoblot was developed using anti-His antibody. d 32Pi transport assays were measured using reconstituted proteoliposomes, showing that YfkE imports Pi in the presence of 0.5 mM Ca2+, not in the presence of 2 mM EDTA. Error bars represent standard deviations (n = 3).
