Fig. 5: Effects of Gdf15 deficiency on gut mucosa during renal injury. | Communications Biology

Fig. 5: Effects of Gdf15 deficiency on gut mucosa during renal injury.

From: Stress-responsive Gdf15 counteracts renointestinal toxicity via autophagic and microbiota reprogramming

Fig. 5

a Eight-week-old wild-type and Gdf15 knockout (KO) mice (n = 3-5) were treated with vehicle or cisplatin (CP; 20 mg/kg) for 72 h. Staining of ileum mucosa with Alcian blue (Magnification, 200×; Scale bar(s), 100 μm) and its quantitation (Magnification, 200×; Scale bar(s), 100 μm). b–d Eight-week-old wild-type and Gdf15 KO mice (n = 3–5) were treated with vehicle or 3% DSS for 8 days. Staining of ileum mucosa with Alcian blue (Magnification, 200×; Scale bar(s), 100 μm) and its quantitation (Magnification, 200×; Scale bar(s), 100 μm) (b). Representative images of the intestinal mucosal bacteria using Gram staining (c) or 16 rRNA in situ staining (d) (Magnification, 200×; Scale bar(s), 100 μm). The mucosal layer thickness was measured (the right graph). The quantitation analysis was shown as plots with Tukey whiskers and outliers (orange circles). Different letters with bars represent significant differences between groups (p < 0.05). Gdf15, growth differentiation factor 15.

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