Fig. 2: Multiplexed images of cell type markers and GMM probability maps overlaid on virtual H&E. | Communications Biology

Fig. 2: Multiplexed images of cell type markers and GMM probability maps overlaid on virtual H&E.

From: 3D reconstruction of skin and spatial mapping of immune cell density, vascular distance and effects of sun exposure and aging

Fig. 2: Multiplexed images of cell type markers and GMM probability maps overlaid on virtual H&E.The alternative text for this image may have been generated using AI.

a Exampleregion of interest within a multiplexed image from Donor 9 (region 3); GMM was used to automatically segment/classify different cell types. Sub-regions of interest are shown in b–d panels with probability maps for each cell type; b cytokeratin CK26 epidermis staining and adjacent probability map of epidermis cells overlaid on the virtual H&E; c endothelial cells of blood vessels (CD31) and adjacent probability map of endothelial cells; d p53 staining in the epidermis and adjacent probability map of p53 positive cells; e The sensitivity, specificity, and accuracy metrics for the cell classification workflow for nine biomarkers using manual annotations.

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