Fig. 2: Tox4 loss impairs proliferation and increases apoptosis of CD8 cells.

a, c Representative plots of flow cytometric analyses of Ki67 expression for cell proliferation in thymocytes (a) and lymphatic T cells (c). b, d Bar graphs comparing frequency of Ki67 positive cells of T cell subpopulations in the thymus (b) and the lymph nodes (d) in control and cKO mice. e, h Representative plots of flow cytometric analyses of 7-AAD and Annexin V staining for apoptosis in thymocyte (e) and lymphatic T cells (h). f, i Bar graphs comparing frequency of early apoptotic (Annexin V⁺7-AAD^-) cells of T cell subpopulations in the thymus (f) and the lymph nodes (i) in control and cKO mice. g, j Bar graphs comparing frequency of late apoptotic (Annexin V⁺7-AAD⁺) cells of T cell subpopulations in the thymus (g) and the lymph nodes (j) in control and cKO mice. Pictures in a, c, e and h are representative of five independent experiments (n = 5). Statistical significance was determined with a two-sided Student’s t-test; the centers and the error bars represent the mean and the SD, respectively. NS: P ≥ 0.05, *P < 0.05, **P < 0.01, and ***P < 0.001.