Fig. 6: Tox4 may restrict elongation but facilitate reinitiation in murine DP cells.
From: Tox4 regulates transcriptional elongation and reinitiation during murine T cell development
a, d, g Genome-wide meta-gene profiles and heatmaps of CUT&Tag comparing chromatin occupancy of total Pol II (a), Pol II (Ser-2p) (d), and Pol II (Ser-5p) (g) in cKO versus control cells. b, c Volcano plots showing Pol II occupancy changes near TSSs (b) and TESs (c) of Tox4-bound genes in cKO versus control cells. e, f Volcano plots showing Pol II (Ser-2p) occupancy changes near TSSs (e) and TESs (f) of Tox4-bound genes in cKO versus control cells. h, i Volcano plots showing Pol II (Ser-5p) occupancy changes near TSSs (h) and TESs (i) of Tox4-bound genes in cKO versus control cells. j Normalized read distribution of CUT&Tag of Tox4, total Pol II, Pol II (Ser-2p), and Pol II (Ser-5p) within the Rmnd5a, Cd8a, Dapk3, and Cdkn2d loci in cKO versus control cells. k, l Comparison of free (k) and chromatin-bound (l) Pol II in control and cKO cells by Western blot. Left: representative pictures of Western blot; Right: a bar graph comparing relative level of total Pol II, Pol II (Ser-2p), Pol II (Ser-5p), and Tox4 in control and cKO cells quantified by ImageJ. β-Actin and H3 were the loading controls for free and chromatin-bound proteins, respectively. Pictures in k and l are representative of three independent experiments (n = 3). Statistical significance was determined with a two-sided Student’s t-test; the centers and the error bars represent the mean and the SD, respectively. NS: P ≥ 0.05, *P < 0.05, **P < 0.01, and ***P < 0.001.
