Fig. 5: RNA-seq of chromosomal CRISPRi targeting shows on-target downregulation and expected downstream effects of transcription factor repression. | Communications Biology

Fig. 5: RNA-seq of chromosomal CRISPRi targeting shows on-target downregulation and expected downstream effects of transcription factor repression.

From: Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects

Fig. 5

Normalized, genome-wide RNA-seq alignment data are shown for 10/84 dcas9 with cyl CRISPRi (A) and A909 dcas9 with covR CRISPRi (B). Non-targeted sham p3015b control strains (black histograms) were used for comparison to targeted strains (red histograms) in both cases. Triplicate biologic replicates, grown to O.D.600 = 1.2, were RNA sequenced from all four strains; alignment data is from all three replicates combined. Log2 fold-change data are shown in which the experimental and control alignments were compared. The zoomed insets display the region of CRISPRi targeting, with the site of the protospacer complement indicated by violet triangles. For A909 dcas9 with covR CRISPRi, orthologous gene expression from RNA-seq was compared to published data defining the covR operon in GBS strain BM11071 (C, D).

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