Fig. 8: Virulence phenotypes are equivalent in an adult mouse sepsis model with CNCTC 10/84 Cas9 variants. | Communications Biology

Fig. 8: Virulence phenotypes are equivalent in an adult mouse sepsis model with CNCTC 10/84 Cas9 variants.

From: Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects

Fig. 8

Adult BALB/cJ female mice (n = 7–8) who received intraperitoneal injection of 2 × 108 CFU of GBS were monitored for death or demonstration of predefined humane endpoints. None of the infected mice died spontaneously, but multiple met the endpoint of ≥20% weight loss from baseline and are shown as nonviable prior to planned dissection at 48 h (A, Mantel-Cox test; B, one-way ANOVA with Bonferroni correction for multiple pre-planned comparisons diagrammed in the legend). Quantification of GBS CFU density in blood, organs, and peritoneal washes collected immediately following euthanasia revealed no significant differences in intraperitoneal persistence or systemic dissemination between pre-planned comparisons (C, one-way ANOVA with Bonferroni correction). NS = not significant.

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