Fig. 6: Fyn-T interacted with ATP synthase.

a Schematic overview of the hybridization of ATP5B with the human proteome microarray. b Replicate experiments of the human proteome microarray of ATP5B-interacting proteins. The Venn diagram indicates the overlap among the candidates of the human proteome microarray. c Proteomic data of the membrane proteins of Jurkat T cells were obtained (Wu et al.⁷⁷) and compared with our proteome microarray data. d The prediction transmembrane protein structure of Fyn-T was analyzed using Membrain. e The plasma membranes of Jurkat T cells were stained by Cell-Mask and probed with the anti-Fyn antibody. Scale bar, 20 μm. BF bright field. f The docking analysis was performed using ClusPro 2.0 and was presented using PyMOL. Human ATP synthase and Fyn-T were modeled using SWISS-MODEL. g, h The best binding models of ATP5A (g) and ATP5B (h) with Fyn-T obtained from docking were analyzed by LigPlot+ 2.2.5 to display the interaction diagrams (partial). The structure of ATP5A and ATP5B are presented on the black line, and Fyn is shown below the black line. Green dotted lines show hydrogen bond interactions. Red and pink semicircles indicate the hydrophobic interactions.