Fig. 2: H2S inhibits recombinant Kv1.5.
a, b Examples of time-series plots illustrating normalized peak current amplitudes evoked by step depolarizations from −70mV to +50 mV in HEK293 cell expressing hKv1.5 before, during (horizontal bar) and after application of the fast-releasing H2S donor NaHS. or the slow-releasing H2S donor GYY4137 via perfusate. Insets show example currents under the conditions described; scale bars: 2 nA (vertical) and 50 ms (horizontal). c, d Mean ( ± s.e.m., values from 4–7 cells), concentration-response relationship illustrating the effects of H2S donor NaHS or GYY4137 on hKv1.5 expressed in HEK 293 cells. Inhibition was determined using currents evoked by step-depolarizations from −70mV to +50 mV. Curves obtained by fitting data to Boltzmann function. e Mean ( ± s.e.m.; n = 9; *P < 0.05) current-density versus voltage relationships before (open circles) and during (solid circles) exposure to NaHS (300 µM). f Families of currents evoked in a HEK293 cell stably expressing hKv1.5 before (upper) and during exposure to NaHS (300 µM; lower). Currents evoked by step-depolarizations, applied up to +80 mV in 10 mV increments from a holding potential of −70 mV. Scale bars apply to both families of currents 2 nA (vertical) and 200 ms (horizontal).
