Fig. 1: Rosemary extract negative-shifts the voltage dependence of homomeric KCNQ2 and KCNQ3 channels.
a Salvia rosmarinus leaves (image: Bo Abbott, used by permission). b Salvia rosmarinus flowers (image: GWA). c Mean traces for water-injected oocytes in the absence (Control) or presence of 1:100 RAP extract. Dashed lines indicate zero current line here and throughout. Scale bars lower left for each trace; voltage protocol upper inset; n = 5 per group. d Mean traces for KCNQ1–3 homomers expressed in oocytes in the absence (Control) or presence of 1:100 RAP extract. Scale bars lower left for each trace; voltage protocol upper inset; n = 5–10 per group. e Mean tail current for traces as in (d); n = 5–10 per group. f Mean normalized tail current (G/Gmax) for traces as in (d); n = 5–10 per group. g Mean unclamped oocyte membrane potential for KCNQ1–3 homomer-expressing oocytes as in d; n = 5 per group. h Mean current fold-increase versus membrane potential for traces as in d in response to 1:100 RAP extract; n = 5–10 per group. i Mean ΔV0.5 activation for traces as in (d) in response to 1:100 RAP extract; n = 5–10 per group. Error bars indicate SEM. n indicates number of biologically independent oocytes. Statistical comparisons by paired t-test or one-way ANOVA. RAP rosemary aerial parts.
