Fig. 6: Hesperidin weakly activates KCNQ2/3 but not KCNQ2. | Communications Biology

Fig. 6: Hesperidin weakly activates KCNQ2/3 but not KCNQ2.

From: Ancient medicinal plant rosemary contains a highly efficacious and isoform-selective KCNQ potassium channel opener

Fig. 6

a Jmol plots of structure (upper) and surface charge (lower) for rosemary compounds as indicated. b Mean traces for KCNQ2/3 heteromers expressed in oocytes in the absence (Control) or presence of 30 or 100 µM rosemary compounds (red) as indicated. Scale bars lower left; voltage protocol upper inset; n = 4-5 per group. c Mean tail current for KCNQ2/3 traces as in (b); n = 4–5 per group. d Mean normalized tail current (G/Gmax) for KCNQ2/3 traces as in (b); n = 4-5 per group. e Mean unclamped oocyte membrane potential for KCNQ2/3-expressing oocytes as in b; n = 4–5 per group. (f) Mean traces for KCNQ2 homomers expressed in oocytes in the absence (Control) or presence of 30 µM hesperidin (red). Scale bars lower left; voltage protocol upper inset; n = 4 per group. (g) Mean tail current for KCNQ2 traces as in (f); n = 4 per group. (h) Mean normalized tail current (G/Gmax) for KCNQ2 traces as in (f); n = 4 per group. i Mean unclamped oocyte membrane potential for KCNQ2-expressing oocytes as in (f); n = 4 per group. Error bars indicate SEM. n indicates number of biologically independent oocytes. Statistical comparisons by one-way ANOVA.

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