Fig. 7: Carnosic acid opens heteromeric KCNQ2/5, KCNQ3/5 and KCNQ2/3/5 channels.
a Mean traces for neuronal KCNQ5-containing homomers and heteromers as indicated, expressed in oocytes in the absence (Control) or presence of carnosic acid (100 µM) (red). Scale bars lower left; voltage protocol upper inset; n = 5 per group. b Mean tail current for channels as in panel a; n = 5 per group. c Mean normalized tail current (G/Gmax) for channels as in panel a; n = 5 per group. d Mean unclamped oocyte membrane potential for oocytes expressing channels as in panel A; n = 5 per group. e Mean ΔV0.5act versus [carnosic acid] for channels as indicated; n = 5 per group; KCNQ3* data from Fig. 4C for comparison. f. Mean ΔEM versus [carnosic acid] for unclamped oocytes expressing channels as indicated; n = 5 per group. KCNQ3* data calculated from Fig. 4D for comparison. g Mean ΔV0.5act versus [carnosic acid] for channels as indicated; n = 5 per group; KCNQ2, KCNQ3 and KCNQ2/3* data from Fig. 4 included for comparison. h Mean ΔEM versus [carnosic acid] for unclamped oocytes expressing channels as indicated; n = 5 per group. KCNQ2, KCNQ3* and KCNQ2/3 data from Fig. 4 included for comparison. Error bars indicate SEM. n indicates number of biologically independent oocytes. Statistical comparisons by t-test or one-way ANOVA.
