Fig. 1: NFI factor motifs are enriched in adult erythroblasts. | Communications Biology

Fig. 1: NFI factor motifs are enriched in adult erythroblasts.

From: Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor

Fig. 1

a Sorting schema for primary erythroblasts derived from CD34 + BM and CB cells to obtain seven discrete cell populations based on the expression of erythroid surface markers CD36 and CD235a. b Analysis of ATAC-seq peaks with increased chromatin accessibility in BM-derived cells relative to CB-derived cells showing enrichment of three NFI factor DNA binding motifs numerically annotated as 1438 [TGGCANNNTGCCA], 2339 (TGCCAA), and 2340 (GCCAA). Asterisks signify Benjamini-Hochberg corrected P-values from a Fisher’s exact test that are ≤ 0.01. c Transcription factor footprinting analysis of differentially accessible peaks between BM- and CB-derived populations 3–5 measuring the difference in footprint depth or flanking accessibility, between BM and CB cells at each TF motif, confirming enrichment of NFI motifs in BM populations. Data are representative of two biological replicates.

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