Fig. 4: NFIX overexpression in CB cells represses HbF. | Communications Biology

Fig. 4: NFIX overexpression in CB cells represses HbF.

From: Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor

Fig. 4

a RT-qPCR validation of NFIX overexpression in CB cells shows approximately 10-fold increase in NFIX transcripts relative to empty vector control cells. Bars represent mean of two biological replicates. b Western blot validation of NFIX overexpression in CB cells as determined by detection of the FLAG tag on day 10 of erythroid differentiation. c CB cells overexpressing NFIX have a reduction in HBG mRNA levels relative to other beta-like globins on day 7 of erythroid differentiation. Bars represent mean of two biological replicates. d CB cells overexpressing NFIX show reduced percentage of F-cells relative to empty vector control on day 14 of erythroid differentiation. e HPLC profiles demonstrate reduction in HbF protein in NFIX overexpressing CB cells relative to the empty vector control on day 14 of erythroid differentiation. Data are representative of N = 2 biological replicates using distinct CB donors and independent NFIX transductions.

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