Fig. 4: Effect of resR/mcdR depletion on metabolic profile of Mtb.
a Heatmap representation of the expression levels of different metabolic genes. Genes associated with respiration, protein translation and lipid metabolism exhibiting differential expression by ≥2-fold (p < 0.05) in three biological repeat experiments are shown. b Heatmap analysis of metabolites. Level of different metabolites was estimated in the empty vector control (blue), resR/mcdR(−) (red) and resR/mcdR(−)::resR/mcdR (green) strains of Mtb mc2 7902 by LC-MS/MS. The heatmap represents normalized abundance of metabolites that are modulated by ≥1.3-fold (p < 0.05) in resR/mcdR(−) compared to control and resR/mcdR(−)::resR/mcdR complemented strains across six biological repeats.
