Fig. 1: Screening of candidate genes responsible for endocrine resistance in human ER+ breast cancer. | Communications Biology

Fig. 1: Screening of candidate genes responsible for endocrine resistance in human ER+ breast cancer.

From: SERPINA3-ANKRD11-HDAC3 pathway induced aromatase inhibitor resistance in breast cancer can be reversed by HDAC3 inhibition

Fig. 1: Screening of candidate genes responsible for endocrine resistance in human ER+ breast cancer.

a Estrogen deprivation for 14 days reduced colony formation of MCF-7 and T47D cells but not their LTED cells. b Estrogen deprivation for 5 days induced a clear reduction in cell proliferation of MCF-7 and T47D but not the LTED cells, as analyzed by cell counting assay. c Flowchart depicts how the 9 candidate genes were selected from various sets of RNA-seq or microarray data. d, e QPCR confirmed the uniform downregulation of the 7 genes in both MCF-7 and T47D LTED cells as compared with their parent cells. E2 estradiol, OVAR ovariectomy-resistance. Data are representative of n = 3 biologically independent experiments and presented as mean ± SD; statistical significance is determined by unpaired Student’s t test (p < 0.05*, p < 0.01**, p < 0.001***).

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