Fig. 1: The organization of Cep63 and Cep152 at the interphase PCM.
a, b 3D-SIM analyses for U2OS cells stably expressing the indicated Cep63 or Cep152 constructs after depleting respective endogenous Cep63 or Cep152 by RNAi. a Representative images displaying the PCM-localized mCherry-Cep63-mGFP or mGFP-Cep152-mCherry signals are shown with two surface-rendered models (top and side views). Original fluorescence images are provided in Supplementary Fig. 1a, b. Boxes, areas of enlargement; double arrows, the diameters and heights measured for quantification. b Quantification of the mCherry and mGFP fluorescent signals in (a) to determine the peak-to-peak diameters (top) and heights (bottom) for the cylindrically localized mCherry-Cep63-mGFP (total n = 53 and n = 48, respectively) and mGFP-Cep152-mCherry (total n = 49 and n = 48, respectively) obtained from three independent experiments. Error bars, mean of n ± s.d. *P < 0.05, ****P < 0.0001 (unpaired two-tailed t test). Detailed methods employed for quantification are described in Supplementary Fig. 1c, d. c (left) A schematic showing the structures of Cep63 and Cep152 with various lengths of CCs (round bars) predicted by the COILS server76. c (right) The organization of Cep63 and Cep152 around a centriole, illustrated based on prior observations12,13,16,27,40,43. Numbers (red and green) indicate the diameters from (b).
