Fig. 4: Topography of the Cep63•Cep152 complex detected by AFM. | Communications Biology

Fig. 4: Topography of the Cep63•Cep152 complex detected by AFM.

From: Architectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in human cells

Fig. 4: Topography of the Cep63•Cep152 complex detected by AFM.

a Surface topographic rendering of the His-Cep63 (424–541)•Cep152 (1205–1295) (left) and His-Cep63 (424–541)•Cep152 (1205–1295) 2D (right) complexes on APS-mica surface. b Quantification of the dimension of the two complexes in (a) from two independent experiments [for His-Cep63 (424–541)•Cep152 (1205–1295): average length = 14.74 ± 1.25 nm and average height = 1.58 ± 0.18 nm (average volume = 123.32 ± 17.47 nm3); for His-Cep63 (424–541)•Cep152 (1205–1295) 2D: average length = 18.35 ± 1.73 nm and average height = 2.16 ± 0.21 nm (average volume = 280.01 ± 45.38 nm3]. Bars, mean of n ± s.d. ****P < 0.0001 (unpaired two-tailed t test). Note that the lateral dimensions of the particles are tip-broadened, while their height is a precise value. Examples of unprocessed “raw” images are provided in Supplementary Fig. 4.

Back to article page