Fig. 5: 3D-SIM (top) and surface rendering (bottom) showing the in vitro self-assemblies generated by the indicated Cep63•Cep152 complex.
a Cylindrical self-assemblies were generated by placing 10 µL of Cep63 (424–541)•Cep152 (1205–1295) (left), Cep63 (424–541)•Cep152 (1205–1272) lacking the 23 basic CC residues (∆23) (middle), and Cep63 (424–541)•Cep152 (1205–1295) (2D) (right) (5 µM) on a poly-l-lysine-coated coverslip. The assemblies on the coverslip were reacted with FITC, washed, and subjected to 3D-SIM as described in the Methods. Boxes, areas of enlargement; arrows, incomplete cylindrical assemblies. b The total number of cylindrical self-assemblies present in the surface area of 1 mm2 was estimated from 15 randomly chosen fields (6813 µm2/field) obtained from three independent experiments (n = 5 fields/sample/experiment). ****P < 0.0001 (unpaired two-tailed t test). Bars, mean of n ± s.d. c The percentage of cylindrical assemblies with a complete circumferential wall quantified from (b). *P < 0.05, ****P < 0.0001 (unpaired two-tailed t test). Bars, mean of three expriments ± s.d.
