Fig. 1: FAM201A over-expression represses NB cell proliferation, migration and invasion.

a Decreased expression of FAM201A expression was detected in NB tissues (NBC, n = 23) and adjacent normal tissues (NBP, n = 23) by Q-PCR analyses; b Q-PCR analysis showed the expressions of FAM201A in CHLA15, CHLA136, SH-N-SH, SH-SY5Y, SK-N-BE(2), SK-N-BE(2)C, and SK-N-AS cell lines; c and d SK-N-BE (2)C and SH-SY5Y cells were transfected with FAM201A over-expression vectors and a empty vector (OC represents over-expression control vectors), and the over-expression effect was verified by Q-PCR analysis; e and f Over-expression of FAM201A inhibited both SK-N-BE (2)C and SH-SY5Y cell proliferation, as shown by CCK8 assays; g–j The number of colonies decreased dramatically after over-expression of FAM201A in the SK-N-BE (2)C and SH-SY5Y cells; k–n Over-expression of FAM021A decreased SK-N-BE (2)C and SH-SY5Y cell proliferation, as shown by EdU assays, magnification, ×200, scale bar, 300 μm; o–r Transwell migration and matrigel invasion assays were used to determine the cell migration and invasion capabilities of SK-N-BE (2)C and SH-SY5Y cells transfected with the over-expression of FAM201A and empty vectors. Shown are quantitation of cell numbers from three independent experiments, magnification, ×100, scale bar, 300 μm. Results in (c–r) are mean ± SD (n = 3). Statistical analysis was done by Student’s t test. (**p < 0.01, ***p < 0.001).