Fig. 5: NBASP regulates FABP5 via ubiquitin proteasome pathway. | Communications Biology

Fig. 5: NBASP regulates FABP5 via ubiquitin proteasome pathway.

From: FAM201A encodes small protein NBASP to inhibit neuroblastoma progression via inactivating MAPK pathway mediated by FABP5

Fig. 5

a and b Western blotting of NBASP protein expression upon over-expression and knockout of the FAM201A, ORF1, and ORF1-mutant in NB cells; c and d Down-regulation of NBASP was confirmed in tumor tissues when compared with adjacent normal tissues (n = 6) at the protein level by western blotting; e The interaction between NBASP and FABP5 was confirmed by a co-immunoprecipitation assay; f Western blotting was performed to detect FABP5 level in ORF1 over-expression and control group after 10 μm CHX treatment for 0 h, 4 h, 8 h; g Western blotting was performed to detect FABP5 level in ORF1 over-expression and control group after 10 μm MG132 treatment for 0 h, 4 h, 8 h; h Co-IP showed ORF1 over-expression bound to more ubiquitin than control group; i Down-regulation of FABP5 has no effect on NBASP. Statistical analysis was done by Student’s t test. (**p < 0.01).

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