Fig. 2: AKAP150 increased in the vlPAG in the CPA model.

Immunofluorescence images (a) and western blotting assay (b) showed that the expression of AKAP150 increased obviously in the pinch pain CPA model. Scale bar, 50 μm (n = 3). (***P < 0.001 vs. the control group, two-sample t test, n = 6). Immunofluorescence images (c) and western blotting assay (d) showed that the expression of AKAP150 increased obviously in the morphine-withdrawal CPA model. Scale bar, 50 μm (n = 3). (***P < 0.001 vs. the control group, two-sample t test, n = 6). e Schematic of the vlPAG in different layers of the mesencephalon. Data are shown as the mean ± SEM.