Fig. 5: TRPV1 in GABAergic neurons of the vlPAG in CPA mice. | Communications Biology

Fig. 5: TRPV1 in GABAergic neurons of the vlPAG in CPA mice.

From: Selective activation of AKAP150/TRPV1 in ventrolateral periaqueductal gray GABAergic neurons facilitates conditioned place aversion in male mice

Fig. 5

Expression of TRPV1 in the pinch pain CPA model (a) and morphine withdrawal CPA model (b). (two sample t test, n = 6). p-TRPV1 was upregulated in the pinch pain CPA model (c, d) and morphine withdrawal CPA model (e, f) [immunofluorescence image (c, e), scale bar, 50 μm (n = 3); western blotting (d, f), ***P < 0.001 vs. the control group, two sample t test, n = 6]. g Expression of TRPV1 after Vgat1-TRPV1-shRNA in WT mice (**P < 0.01 vs. Vgat1-shRNA-NC group, two sample t test, n = 6). Vgat1-TRPV1-shRNA treatment in mice [CPA tests for pinch pain CPA model (h) and morphine withdrawal model (k) mice, ***P < 0.001 vs. the Vgat1-shRNA-NC group, two sample t test, n = 10; immunofluorescence image (i, l), scale bar, 50 μm; n = 3; western blotting assay (j, m), n.s. No significance, ***P < 0.001 vs. the Vgat1-shRNA-NC group, two sample t test, n = 6]. Data are shown as the mean ± SEM.

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