Fig. 3: ROS generation by IP-opsonized merozoites in blood phagocytes depends on nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX2). | Communications Biology

Fig. 3: ROS generation by IP-opsonized merozoites in blood phagocytes depends on nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX2).

From: Human blood neutrophils generate ROS through FcγR-signaling to mediate protection against febrile P. falciparum malaria

Fig. 3

PBLs were incubated with immune plasma (IP) or non-immune plasma (NP) plus 2.5 µM diphenyleneiodonium (DPI, an inhibitor of NOX2) and/or 500 µM 4-aminobenzoic acid hydrazide (ABAH, an inhibitor of myeloperoxidase (MPO)). Graphs show the relative DCF or isoluminol signal of neutrophils (a), monocytes (b), and PBLs (c) using the untreated cells (IP) as reference. Horizontal lines represent median values. P values were determined by the Friedman test and Dunn’s multiple comparisons test. The values shown are from a single independent experiment (n = 12).

Back to article page