Fig. 4: IP-opsonized ROS generation in blood phagocytes depends on the phosphoinositide 3-kinase signaling pathway.

PBLs were incubated with immune plasma (IP) or non-immune plasma (NP) plus 250 nM Gö6983 (an inhibitor of protein kinase C) and/or 100 nM wortmannin (an inhibitor of phosphoinositide 3-kinase). Graphs show the relative DCF or relative area under curve (AUC) of isoluminol chemiluminescence signal of neutrophils (a), monocytes (b), and PBLs (c) using the untreated cells (IP) as reference. Horizontal lines represent median values. P values were determined by the Friedman test and Dunn’s multiple comparisons test. The values shown are from a single independent experiment (n = 12).