Fig. 3: Assessment of task-driven behaviour in delirium mice.

a Schematic diagram of a novel object recognized under multi-angle shooting. Schematic diagram of the test. The orange quadrangular prism is a familiar object, which is an object that was repeatedly touched before the test. The green cone represents objects that are touched for the first time, namely new objects. For detecting the replacement of new objects in daily behaviour, see the Methods and Supplementary Figure 3. b Comparison of the fraction of task-driven movement types between non-POD mice and POD mice in NOR. The bold traces and shadows indicate the mean ± SEM (blue, non-POD, n = 33; pink, POD, n = 19). Two movements showed significant differences between the two groups, that POD mice prefer is sniffing (M35, non-POD = 0.028 ± 0.004, POD = 0.09 ± 0.039), less is left looking (M39, non-POD = 0.095 ± 0.011, POD = 0.053 ± 0.011) ****M35, P < 0.0001; ****M39, <0.0001. c Comparison of the fraction of movement types between non-POD mice and POD mice for exploring the novel objects. The bold traces and shadows indicate the mean ± SEM (blue, non-POD, n = 33; pink, POD, n = 19). Two movements showed significant differences between the two groups, and the fractions of the four movements that POD mice prefer are stretching ahead (M20, non-POD = 0.027 ± 0.009, POD = 0.074 ± 0.032), less is left looking (M39, non-POD = 0.096 ± 0.023, POD = 0.052 ± 0.018). *M20, P = 0.0127; *M39, P = 0.0255. d Comparison of the fraction of movement types between non-POD mice and POD mice for exploring a familiar object. The bold traces and shadows indicate the mean ± SEM (blue, non-POD, n = 33; pink, POD, n = 19). Three movements showed significant differences between the two groups, and the fractions of the four behaviours that POD mice prefer are stretching ahead (M20, non-POD = 0.037 ± 0.11, POD = 0.095 ± 0.032) and walking with head down (M31, non-POD = 0.017 ± 0.004, POD = 0.067 ± 0.052), less is right looking (M40, non-POD = 0.073 ± 0.018, POD = 0.028 ± 0.007). ***M20, P = 0.0006; *M31, P = 0.016; *M40, P = 0.0374. e Schematic diagram of the different behaviours. f We mapped the AcSeq to distinguish deep-seeking behaviour and shallow seeking behaviour in mice. g Probability histogram and bar graph of the DSP index of POD mice exploring familiar and novel objects. The deep versus shallow investigation preference (DSP) varies between -π/2 and π/2, where -π/2 and π/2 indicate the absolute preference for shallow and deep investigation, respectively, and 0 indicates an equal preference for deep and shallow investigation. Probability histogram and bar graph of the DSP index of POD mice(n = 19) exploring familiar and novel objects (Familiar = −0.359 ± 5.84, Novel = 8.854 ± 7.810). Statistics: (Mann–Whitney test; NS not significant, P = 0.323). h Probability histogram and bar graph of the DSP index of non-POD mice exploring familiar and novel objects (Familiar = 2.639 ± 3.59, Novel=14.02 ± 3.84). Statistics: (Mann–Whitney test; *P = 0.0340). All data were presented as mean ± SEM.