Fig. 5: Freezing profiles in four strains of Limnomonas gaiensis to investigate the nature of ice nucleation active compounds and the temperature spectra at which this reaction occurs. | Communications Biology

Fig. 5: Freezing profiles in four strains of Limnomonas gaiensis to investigate the nature of ice nucleation active compounds and the temperature spectra at which this reaction occurs.

From: Aerosolization flux, bio-products, and dispersal capacities in the freshwater microalga Limnomonas gaiensis (Chlorophyceae)

Fig. 5

The four investigated strains of Limnomonas gaiensis, R86-45 (a), R86-47 (b), VR66-07 (c), and VR66-10 (d) exposed to a decreasing gradient of −1 °C from −4 to −21 °C. The total fraction is indicated in green. Treatments included either protein denaturation by heat (non-proteinaceous, orange), molecule size selection by filtration on 0.22 µm pore membrane (soluble, blue), or a combination of both treatments (soluble non-proteinaceous, purple). The number of replicates was of 32 droplets of 20 µL of either microalgae (strain), MWC (negative control, black) or Pseudomonas syringae active >-4 °C (positive control, gray). The error bars show the 95% confidence interval. Each data point is the synthesis of a total of 52 to 64 replicates per strain and treatment, and of 116 replicates per control.

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