Fig. 5: Temporal control of endogenous gene expression mediated by morpholinos. | Communications Biology

Fig. 5: Temporal control of endogenous gene expression mediated by morpholinos.

From: Ribo-On and Ribo-Off tools using a self-cleaving ribozyme allow manipulation of endogenous gene expression in C. elegans

Fig. 5: Temporal control of endogenous gene expression mediated by morpholinos.The alternative text for this image may have been generated using AI.

a Diagram representing how an antisense oligonucleotide inactivates a ribozyme to restore protein expression and relative controls. Adapted with permission from Wurmthaler et al.11. b Confocal images to show the morphology of mitochondria in wild-type and mutant animals. Scale bars: 20 μm. c Diagram showing the cassette (outlined by a box) integrated into the C-terminal of argn-1. d A workflow of injecting morpholinos into the worm germ line and confocal imaging for quantifications. e Fluorescence images to show the morphology of mitochondrial and ARGN-1 expression under various conditions. Scale bar, 20 μm. f Relative protein expression of ARGN-1::GFP under different conditions as shown in (e). Each dot represents a single worm. Number of animals quantified: n = 35 for ARGN-1::GFP; n = 24 for ARGN-1::GFP::Rz*; n = 19 for ARGN-1::GFP::Rz without v-M8; n = 27 for ARGN-1::GFP::Rz injected with 50 μM v-M8; n = 21 for ARGN-1::GFP::Rz injected with 100 μM v-M8; n = 18 for ARGN-1::GFP::Rz injected with 200 μM v-M8; n = 21 for ARGN-1::GFP::Rz injected with 250 μM v-M8; n = 39 for ARGN-1::GFP::Rz injected with 400 μM v-M8. All data were shown as mean ± s.e.m. Ns: not significant. *p < 0.05. ****p < 0.0001 (one-way ANOVA with the Tukey correction). g Quantifications of mitochondria morphology under different conditions as shown in (e). The number of animals quantified for each group was indicated above each column. h The expression of ARGN-1::GFP was measured based on fluorescent imaging at indicated time points (hours after V-M8 morpholino or M9 injection) for various genetic backgrounds (GFP, GFP::Rz* or GFP::Rz). All values are displayed as mean ± s.e.m. n ≥ 19 animals. The exact number of animals is provided as source data. i The percentage of GFP-Rz animals containing normal mitochondrial was quantified at indicated time points after V-M8 morpholino or M9 injection. n ≥ 26 animals. The exact number of animals is provided as source data. Arrows in (b) and (e): enlarged mitochondria. Source data are provided as a Source Data file.

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